Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Sodium Cacodylate Buffer (0.1 M, pH 6.5) - 100ml
Sodium Cacodylate Buffer (0 1 M pH 6 5) - 100ml (For Research Use and Further Manufacturing Only)
Beckman Coulter Flow-Check Pro Fluorospheres
Flow-Check Pro Fluorospheres is a suspension of fluorescent microspheres used for daily verification of the Navios™or FC 500 Flow Cytometer optical alignment and fluidics system.
Alkali Scientific QuickSilver™ PBS Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Research Products International Corp Potassium Phosphate, Dibasic, 10 KG
Potassium Phosphate Dibasic, Anhydrous (dipotassium phosphate) is a buffering agent commonly used in molecular biology, biochemistry, and cell culture. It effectively maintains pH in the neutral to slightly alkaline range (7.0–8.0), making it ideal for applications such as protein purification, enzyme assays, and cell culture media. Its strong buffering capacity ensures experimental consistency. In addition to pH control, it serves as a potassium ion source—supporting cellular processes and metabolism. Its dual role as a pH stabilizer and nutrient makes it valuable in media formulation and biochemical research.
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660121.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are designed for laboratories that need to prepare larger volumes of PBS quickly and accurately. Each tablet dissolves easily in water to create 200 mL of phosphate-buffered saline, a standard buffer used in many biological and biochemical applications. The 500-tablet bottle is perfect for high-throughput labs that require a consistent and reliable buffer solution. These tablets ensure that the PBS solution maintains the correct pH and osmolarity, which is essential for applications such as cell culture, protein assays, and molecular biology experiments. The tablets are convenient, cost-effective, and reduce the chance of errors in solution preparation.
Ethos Biosciences Dilurex 10X PBS Buffer, 1000ml, Ethos
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution. Save space by keeping 10x concentrate and dilute to 1x pH 7.4 working concentration as needed by mixing 1 part 10X PBS with 9 parts distilled water. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
Quality Biological Inc HBSS without Phenol Red, 500 mL
Hanks¿ Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. ¿ Provides cells with a source of water, essential inorganic ions, and carbohydrate as an energy source. ¿ Useful for diluting cells for counting. ¿ Formulations with calcium and magnesium support cell adhesion and are generally used as transport media, or for reagent preparation. ¿ Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc PBS (10X), pH 7.4, Case 10 X 500 mL
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 100/pk
Phosphate buffered saline | MFCD00131855 | 100/pk
Teknova 1X PBS with 0.1% Tween 20, 1% BSA. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.