Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc HEPES Free Acid 100 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc HEPES Free Acid 5 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc MOPS Free Acid Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 6 5 7 9 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc PBS Tablets
PBS (phosphate buffered saline) is a common buffer solution used in the laboratory It is an isotonic and non-toxic buffer that is meant to mimic the osmolarity and ion concentrations of the human body PBS is used for immunoassays for sample dilution for protein purification and as a wash buffer in cell cultures PBS tablets are premeasured tablets for convenient preparation of 1X PBS solution at a pH of 7 3 7 5 PBS buffer contains 10mM phosphate buffer 137mM sodium chloride and 2 7mM potassium chloride
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
eMolecules TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | 158144-85-5 | MFCD11044987 | 1g
AstaTech | TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | 1g | 206684235 | 81367 | 95.000 | 158144-85-5 | MFCD11044987 | 291.391 | C17H25NO3
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Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)METHYLGLYCI
Tris(hydroxymethyl)methylglycine analytical grade MP 179 - 187 °C; pKa 20 = 8.15. Buffering substance (1). Non-toxic substitute for barbital (2). Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 37195.03 CAS 1389-47-5
Crescent Chemical Co Inc SERVA TRIS-GLYCINE/SDS BUFFER
SERVA Tris-Glycine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 250 mM Tris, 1.92 M glycine, 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42529.01
Crescent Chemical Co Inc TE BUFFER (100X) PH 8.0
TE Buffer (100X) pH 8.0 DNase/RNase not detected. 100 x concentrated aqueous solution. Size - 100ML Storage Conditions - +15 °C TO +30 °C Catalog Number - 39799.01
Bioworld BSA (1%) IN PBS, DNASE AND RNA
BSA (1%) in PBS, DNase and RNase Free0.79% Sodium Chloride, 0.02% Potassium Chloride, 0.06% Sodium Phosphate, 0.02% Potassium Phosphate, 1.0% BSA, DNAse/RNAse-Free
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 25g
Trizma base | Purity: 99.9% | MW: 121.14 | 77-86-1 | MFCD00004679 | 25g
Fluidigm Corp Maxpar PBS, 500 mL
Formulated to provide the best possible signal to noise ratio for detection by mass cytometry.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 20 X 1ml
Crude cell extracts typically contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins During cell or tissue protein extraction in vitro protein synthesis is significantly inhibited while degradation is enhanced Adding inhibitors against these enzymes can improve yields of intact proteins Protease inhibitor cocktails are used with cell lysates or tissue extracts to enhance protein stability Our protease inhibitor cocktail has broad specificity and serves as a general-use protease inhibitor set suitable for purifying histidine-tagged proteins It contains multiple critical components including AEBSF Aprotinin Bestatin E-64 Leupeptin Phosphoramidon and Pepstatin A with broad inhibition specificity against serine proteases cysteine proteases acidic proteases aminopeptidases and metalloproteases The product is supplied as a ready-to-use solution in DMSO and can be used in WB Co-IP pull-down assays IF IHC and kinase assays